Researchers Discover Diabetic Wound Healing Compound

 
Non-healing chronic wounds are a major complication of diabetes, which result in more than 70,000 lower-limb amputations in the United States alone each year. The reasons why diabetic wounds are resistant to healing are not fully understood, and there are limited therapeutic agents that could accelerate or facilitate their repair.

In a new study that appears in the journal Proceedings of the National Academy of Sciences (PNAS), the researchers report the discovery of a better MMP-9 inhibitor referred to as ND-336

A team of researchers from Notre Dame's Department of Chemistry and Biochemistry, led by Mayland Chang, previously identified two enzymes called matrix metalloproteinases (MMPs), MMP-8 and MMP-9, in the wounds of diabetic mice. They proposed that the former might play a role in the body's response to wound healing and the latter was the pathological consequence of the disease with detrimental effects. The researchers used the MMP-9 inhibitor referred to as ND-322.

We found that wounds treated with ND-336 healed significantly faster than those treated with ND-322 because of the better selectivity of ND-336 than ND-322 for inhibition of MMP-9 over MMP-8. In the current paper, we applied the enzyme MMP-8 to wounds of diabetic mice and found accelerated wound healing. We also combined the MMP-9 inhibitor ND-336 and the enzyme MMP-8 and found further acceleration of diabetic wound healing.”

“ND-336 is a six-fold more potent inhibitor than ND-322 and has 50-fold selectivity towards inhibition of MMP-9 than MMP-8,” Chang said. “In contrast, ND-322 has three-fold selectivity towards inhibition of MMP-9 compared to MMP-8. The current paper compared the efficacy of ND-336 versus ND-322.

The researchers found that a combination of a selective inhibitor of MMP-9 (a small molecule) and applied MMP-8 (an enzyme) enhanced healing even more, in a strategy that holds considerable promise in healing of diabetic wounds.